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KMID : 0620720110170030245
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Natural Product Sciences
2011 Volume.17 No. 3 p.245 ~ p.249
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Topoisomerase I and II Inhibitory Activities and Cytotoxic Constituents from the Barks of Tilia amurnesis
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Piao Dong-Gen
Lee You-Jeong
Seo Chang-Seob
Chang Hyun-Wook
Son Jong-Keun
Lee Chong-Soon
Kim Jae-Ryong
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Abstract
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Eight compounds, squalene (1), friedelin (2), B-sitosterol (3), B-sitosterol-3-O-glucoside (4), a-tocopherol (5), betulinic acid (6), trilinolein (7) and 1-O-(9Z,12Z-Octadecadienoyl)-3-nonadecanoyl glycerol (8), were isolated from the barks of Tilia amurensis. Their chemical structures were identified by comparing their physicochemical and spectral data with those published in the literature. These isolated compounds were examined for their inhibitory activities against topoisomerase I and II. Compound 7 showed significant inhibition of DNA topoisomerase I and II activities, with percent decreases in activity of 87 and 95%, respectively at a concentration of 100 uM. Compound 6 exhibited cytotoxicity against the human colon adenocarcinoma cell line (HT-29), the human breast adenocarcinoma cell line (MCF-7) and the human liver hepatoblastoma cell line (HepG-2), with IC50 values of 20, 59 and 16 uM, respectively.
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KEYWORD
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Cytotoxicity, DNA topoisomerases I, DNA topoisomerases II, Tilia amurensis
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