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KMID : 0620720110170040315
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Natural Product Sciences
2011 Volume.17 No. 4 p.315 ~ p.320
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A New HPLC-analytical Method for Total Sphingosine Contents as an Indirect Index for the Ganglioside Contents of Deer Antlers
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Choi Hye-Ok
Kim Jeung-Won
Jo Sung-Jun
Kim Jung-Hwan
Han Byung-Hoon
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Abstract
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Routinely applicable HPLC assay procedures for the ganglioside content in various deer antler preparations were established through the creation of a UV-absorbing chromophoric substance - trans-a,B- unsaturated-hexadecene-aldehyde - from the sphingosine moiety in ganglioside molecules by two step chemical reactions. In order to guarantee the assay`s accuracy and sensitivity, the HPLC-assay procedure adopted internal reference procedures by mixing cis-α,β-unsaturated-hexadecene aldehyde[V] or cis-3-heptadecene- 1,2-diol[IV] to assay samples. The internal reference compound [IV] or [V] was synthesized in our laboratory starting from mannitol-diacetonide through three or four step organic reactions. This new HPLC-assay procedure was successfully applied to deer antler extracts with good dose-dependent calibration curves at the picomole level of gangliosides.
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KEYWORD
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HPLC-sphingosine, deer antler, ossified deer antler, a, B-unsaturated-alkene-aldehyde
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