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KMID : 0620720120180020083
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Natural Product Sciences
2012 Volume.18 No. 2 p.83 ~ p.88
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Quantitative Analysis of Bioactive Compounds in the Fruits of Crataegus pinnatifida by High-Performance Liquid Chromatography
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Bae Yoon-Ho
To Dao Cuong
Woo Mi-Hee
Min Byung-Sun
Lee Jae-Hyun
Choi Jae-Sue
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Abstract
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In order to facilitate the quality control of the fruits of Crataegus pinnatifida, a simple, accurate and reliable HPLC method was developed for the simultaneous determination of the three bioactive compounds: chlorogenic acid (1), rutin (2), and hyperin (3), which were selected as the chemical markers of C. pinnatifida. Separation was achieved on an Agilent Eclipse XDB-C18 column with a gradient solvent system of 0.1% trifluoroacetic acid aqueous-acetonitrile at a flow-rate of 1.0 mL/min and detected at 254nm. All three calibration curves showed good linearity (R2 > 0.998). The recoveries of three marker compounds were in the range of 94.87~111.52 %. The contents of chlorogenic acid (1), rutin (2), and hyperin (3) of the fruits of C. pinnatifida collected from 23 district markets in Korea, Japan, and China were 0.16~0.65 mg/g, 0.07~1.24 mg/g, and 0.03~0.62 mg/g, respectively. The results demonstrated that this method is simple and reliable for the quality control of the fruits of C. pinnatifida.
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KEYWORD
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Fruits of Crataegus pinnatifida, HPLC, Chlorogenic acid, Rutin, Hyperin
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