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KMID : 0620720130190020179
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Natural Product Sciences
2013 Volume.19 No. 2 p.179 ~ p.185
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Ethanol Extract of Polygalae Radix Augments Pentobarbital-Induced Sleeping Behaviors through GABAAergic Systems
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Lee Chung-Il
Lee Mi-Kyeong
Oh Ki-Wan
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Abstract
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Polygalae radix (PR) has traditionally been used as a sedative and anti-stress agent in oriental countries for a long time. PR which contains many ingredients is especially rich in saponins. This study was performed to investigate whether ethanol extract of PR enhances pentobarbital-induced sleep behaviors. In addition, possible mechanisms also were investigated. PR inhibited locomotor activity in mice. PR increased sleep rate and sleep time by concomitant administration with sub-hypnotic dose of pentobarbital (28 mg/kg). PR prolonged total sleeping time, and shortened sleep latency induced by pentobarbital (42 mg/kg). In addition, PR increased intracellular chloride concentration in primary cultured neuronal cells. The expression level of glutamic acid decarboxylase (GAD) were increased, and r-aminobutyric acid (GABA)A receptors subunits were modulated by PR, especially increasing r-subunit expression. In conclusion, PR augments penobarbital-induced sleep behaviors through activation of GABAA receptors and chloride channel complex.
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KEYWORD
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Polygalae radix, Locomotor, Sleeping, Glutamic acid decarboxylase, Chloride influx, GABAA receptors
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