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KMID : 0620720140200020080
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Natural Product Sciences
2014 Volume.20 No. 2 p.80 ~ p.85
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Anti-oxidative Activities of 3`-Hydroxygenkwanin from the Flower Buds of Daphne genkwa in Caenorhabditis elegans
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Park Sung-Hoon
Cui Xun
Ahn Dal-Rae
Lee Eun-Byeol
Cha Dong-Seok
Jeon Hoon
Kim Dae-Keun
Zee Ok-Pyo
Kim Youn-Chul
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Abstract
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In the course of screening for antioxidant compounds from natural plants in Korea by measuring the radical scavenging effect, a methanol extract of the flower buds of Daphne genkwa S. et Z. (Thymelaeaceae) was found to show a potent antioxidant activity. Subsequent activity-guided fractionation of methanol extract of D. genkwa led to the isolation of four compounds from the ethyl acetate soluble fraction. The chemical structures were elucidated as genkwanin (1), 3`-hydroxygenkwanin (2), apigenin (3), and tiliroside (4) by spectroscopic techniques. Among them, compound 2 showed the significant anti-oxidative effect on DPPH. And compound 2 showed the significant riboflavin- and xanthine-originated superoxide quenching activities. To verify the antioxidant enzymatic activities of compound 2, the SOD enzymatic activity was measured spectrophtometrically using prepared Caenorhabditis elegans homogenates. The results showed that compound 2 was able to elevate SOD activity of C. elegans in a dose dependent manner. Moreover, compound 2 decreased the intracellular ROS accumulation of worms.
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KEYWORD
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Daphne genkwa, 3`-Hydroxygenkwanin, Antioxidant, DPPH, Caenorhabditis elegans, SOD, ROS
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