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KMID : 0620720140200020113
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Natural Product Sciences
2014 Volume.20 No. 2 p.113 ~ p.118
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Anti-inflammatory Effect of Ribes fasciculatum in IFN-γ/LPS-stimulated Mouse Peritoneal Macrophage
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Kim Jin-Kyu
Kim Ha-Na
Kang Chung-Sik
Seo Je-Han
Seo Hyun-Won
Im Jun-Sang
Kim Bong-Seok
Cha Dong-Seok
Jeon Hoon
Kwon Jin
Oh Chan-Ho
Ma Sang-Yong
Nam Jung-Il
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Abstract
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Ribes fasciculatum which belongs to Saxifragaceae has been widely used as a traditional medicine for the treatment of symptoms associated with lacquer poison. However, pharmacological studies on the R. fasciculatum are extremely limited until now. Thus, in this study, we evaluated the possible anti-inflammatory effects of ethyl acetate fraction of R. fasciculatum (ERF) using IFN-γ/LPS-stimulated peritoneal macrophage model. We investigated the change in nitrite level in the absence or presence of ERF after LPS stimulation, and we found that ERF effectively attenuates the NO production in a dose dependent manner without notable toxicity. To determine the mechanism of the inhibitory action of ERF on NO production, we performed iNOS enzyme activity assay and Western blotting. Here we showed that both of iNOS enzyme activities and iNOS expressions were significantly down-regulated by ERF, indicating that these dual activities of ERF are responsible for ERFmediated NO suppression. In addition, ERF inhibitied the expression of cyclooxygenase-2 (COX-2), an another key enzyme in inflammation through suppression of NF-κB activation. We also tested anti-inflammatory properties of ERF not only in vitro, but in vivo using trypsin-induced paw edema model in mice. Our results revealed that the increased paw volume in response to trypsin injection was recovered by ERF supplement dose dependently.
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KEYWORD
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Ribes fasciculatum, Inflammation, Nitric oxide, Edema
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