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KMID : 0620720150210030219
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Natural Product Sciences
2015 Volume.21 No. 3 p.219 ~ p.225
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4-Hydroxybenzaldehyde, One of Constituents from Gastrodiae Rhizoma Augments Pentobarbital-induced Sleeping Behaviors and Non-rapid Eye Movement (NREM) Sleep in Rodents
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Choi Jae-Joon
Kwon Yeong-Ok
Yoo Jae-Hyeon
Lee Mi-Kyeong
Hong Jin-Tae
Oh Ki-Wan
Kim Young-Shik
Chong Myong-Soo
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Abstract
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In the previous experiments, we reported that ethanol extract of Gastrodiae Rhizoma, the dried tuber of Gastrodia ElataBlume (Orchidaceae) increased pentobarbital-induced sleeping behaviors. These experiments were undertaken to know whether 4-hydroxybenzaldehyde (4-HBD), is one of the major compounds of Gastrodiae Rhizoma increases pentobarbital-induced sleeping behaviors and changes sleep architectures via activating GABAA-ergic systems in rodents. 4-HBD decreased locomotor activity in mice. 4-HBD increased total sleep time, and decreased of sleep onset by pentobarbital (28 mg/kg and 40 mg/kg). 4-HBD showed synergistic effects with muscimol (a GABAA receptor agonist), shortening sleep onset and enhancing sleep time on pentobarbital-induced sleeping behaviors. On the other hand, 4-HBD (200 mg/kg, p.o.) itself significantly inhibited the counts of sleepwake cycles, and prolonged total sleep time and non-rapid eye movement (NREM) in rats. Moreover, 4-HBD increased intracellular Cllevels in the primary cultured cerebellar cells. The protein levels of glutamic acid decarboxylase (GAD) and GABAA receptors subunits were over-expressed by 4-HBD. Consequently, these results demonstrate that 4-HBD increased NREM sleep as well as sleeping behaviors via the activation of GABAA-ergic systems in rodents.
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KEYWORD
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4-Hydroxybenzaldehyde (4-HBD), Pentobarbital, Intracellular chloride, Glutamic acid decarboxylase, GAD, GABAA receptors subunits
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