|
KMID : 0620720160220010020
|
|
Natural Product Sciences
2016 Volume.22 No. 1 p.20 ~ p.24
|
|
|
Biological Activities and Stability of a Standardized Pentacyclic Triterpene Enriched Centella asiatica Extract
|
|
Panupong Puttarak
Pharkphoom Panichayupakaranant
Adelheid Brantner
|
|
|
Abstract
|
|
|
|
Pentacyclic triterpenes, mainly, asiatic acid, madecassic acid, asiaticoside, and madecassoside are the active constituents of Centella asiatica. A pentacyclic triterpene enriched C. asiatica extract (PRE) was prepared and standardized to contain a total pentacyclic triterpenes not less than 65% w/w. This work was focused on determination of antiinflammatory, antioxidant, and tyrosinase inhibitory activities of PRE and its stability. The PRE exhibited a satisfactory nitric oxide inhibitory effect, with an IC50 value of 64.6 μg/mL. In addition, the PRE inhibited tyrosinase enzyme activity with an IC50 value of 104.8 μg/mL. In contrast, the PRE possessed only weak antioxidant activity. The PRE was stable over a period of four months when stored as a dried powder but only in a well-closed container protected from light at 4 oC. An aqueous alcoholic solution of the PRE was stable at pH values of 5.8 and 7.0, but was not stable at a pH of 8.2. Preparations of the PRE in an aqueous solution should be performed in acidic or neutral conditions.
|
|
|
KEYWORD
|
|
|
Centella asiatica, Pentacyclic triterpenes, Antiinflammatory, Antioxidant, Tyrosinase inhibition, Stability
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|