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KMID : 0620720160220020134
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Natural Product Sciences
2016 Volume.22 No. 2 p.134 ~ p.139
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Phytochemical Identification from Boehmeria nivea Leaves and Analysis of (-)-Loliolide by HPLC
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Cho Sung-Hun
Lee Dong-Gu
Lee Sang-Hyun
Jung Yong-Su
Kim Ho-Bang
Cho Eun-Ju
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Abstract
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Phytochemicals were isolated from leaves of the fiber crop, ramie (Boehmeria nivea, Bn), using open column chromatography and medium pressure liquid chromatography. Their structures were identified as β- sitosterol, (-)-loliolide, rutin, and pyrimidinedione by MS, 1H-, and 13C-NMR spectroscopic analysis. Among them, (-)-loliolide was isolated for the first time from B. nivea. A content analysis of (-)-loliolide in B. nivea collected from different regions and harvest times was conducted by HPLC. The highest content of (-)-loliolide was found in Bn-23 harvested in September. These results will be helpful to use the plant which harvest in September as a high content phytochemical additive in food, health supplements, and medicinal products.
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KEYWORD
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Boehmeria nivea, Urticaceae, Phytochemical, Identification, Loliolide, NMR
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