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KMID : 0620720170230010053
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Natural Product Sciences
2017 Volume.23 No. 1 p.53 ~ p.60
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Ethanol Extract of Perillae Herba Enhances Pentobarbital-Induced Sleep and Non-Rapid Eye Movement (NREM) Sleep through GABAA-ergic Systems
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Kwon Yeong-Ok
Ha Tae-Woo
Oh Ki-Wan
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Abstract
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Perillae Herba has been traditionally used for the sedation in the oriental countries. Therefore, this study was conducted to determine whether Perillae Herba ethanol extract (PHEE) enhances pentobarbital-induced sleeping behaviors in animals. In addition, the possible mechanisms are demonstrated. PHEE (12.5, 25 and 50 mg/kg. p.o.) reduced the locomotor activity in mice. PHEE reduced sleep latency and augmented the total sleep time in pentobarbital (42 mg/kg, i.p.)-induced sleep in mice. Furthermore, the number of sleeping mice treated with sub-hypnotic pentobarbital (28 mg/kg, i.p.) increased. PHEE (50 mg/kg. p.o.) decreased the sleep/wake cycles and wakefulness, and increased total sleeping time and NREM sleep in electroencephalogram (EEG) of rats. In addition, PHEE (0.1, 1.0 and 10 μg/ml) increased the intracellular Cl- level through the GABA receptors in the hypothalamus of rats. Moreover, the protein of glutamate decarboxylase (GAD) was overexpressed by PFEE. It was found that PHEE enhanced pentobarbital-induced sleeping behaviors through GABAA-ergic transmissions.
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KEYWORD
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Perillae Herba ethanol extract (PHEE), Pentobarbital-induced sleep, Glutamic acid decarboxylase (GAD), GABA, GABAA receptors, Electroencephalogram (EEG)
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