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KMID : 0620720170230040247
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Natural Product Sciences
2017 Volume.23 No. 4 p.247 ~ p.252
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Rapid Identification of Methylglyoxal Trapping Constituents from Onion Peels by Pre-column Incubation Method
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Kim Ji-Hoon
Kim Myeong-Il
Syed Ahmed Shah
Jung Ki-Won
Kim Chul-Young
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Abstract
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The methylglyoxal (MGO) trapping constituents from onion (Allium cepa L.) peels were investigated using pre-column incubation of MGO and crude extract followed by HPLC analysis. The peak areas of MGO trapping compounds decreased, and their chemical structures were identified by HPLC-ESI/MS. Among major constituents in outer scale of onion, 2-(3,4-dihydroxybenzoyl)-2,4,6-trihydroxy-3(2H)-benzofuranone (2) was more effective MGO scavenger than quercetin (6) and its 4'-glucoside, spiraeoside (3). After 1 h incubation, compound 2 trapped over 90% MGO at a concentration of 0.5 mM under physiological conditions, but compounds 3 and 6 scavenged 45%, 16% MGO, respectively. HPLC-ESI/MS showed that compound 2 trapped two molecules of MGO to form a di-MGO adduct and compounds 3 and 6 captured one molecule of MGO to form mono-MGO adducts, and the positions 6 and 8 of the A ring of flavonoids were major active sites for trapping MGO.
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KEYWORD
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Allium cepa L., Methylglyoxal trapping, Pre-column incubation, Flavonoids
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