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KMID : 0620720170230040270
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Natural Product Sciences
2017 Volume.23 No. 4 p.270 ~ p.273
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Quantitative Determination of Bakkenolide D in Petasites japonicus and Farfugium japonicum by HPLC/UV
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Quilantang Norman G.
Lee Ki-Ho
Lee Dong-Gu
Lee Ju-Sung
Cho Eun-Ju
Kim Hyun-Young
Lee Sang-Hyun
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Abstract
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A quantitative analysis of bakkenolide D in the different parts of Petasites japonicus and Farfugium japonicum was performed by HPLC. A gradient HPLC elution system with a mobile phase consisting of water: acetonitrile solution (20:80 to 0:100 for 45 min) was followed and an INNO C18 column was used for the chromatographic separation. The injection volume, flow rate, and UV detection were 10 μL, 1 mL/min, and 290 nm, respectively. Results show that both species showed the highest amount of bakkenolide D in the roots being 107.203 and 166.103 mg/g for P. japonicas and F. japonicum, respectively. Content analysis on the different parts of both plants displayed remarkably lower values which ranged from 0.403 - 4.419 and 7.252 - 32.614 mg/g for P. japonicas and F. japonicum, respectively. The results show that the roots of both plants are rich in bakkenolide D showing a promising use in the development of nutraceuticals and industrial application of the compound.
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KEYWORD
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Bakkenolide D, Petasites japonicus, Farfugium japonicum, HPLC/UV
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