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KMID : 0620720180240010036
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Natural Product Sciences
2018 Volume.24 No. 1 p.36 ~ p.39
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Content Analysis of Rutin in the Leaves of Boehmeria nivea Harvested in Different Regions of South Korea by HPLC-UV
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Cho Sung-Hun
Quilantang Norman G.
Lee Ju-Sung
Kim Young-Mi
Kim Ho-Bang
Cho Eun-Ju
Jung Yong-Su
Lee Sang-Hyun
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Abstract
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Phytochemical analysis of Boehmeria nivea (Bn) leaves by medium pressure liquid chromatography led to the isolation of a flavonoid glycoside identified by spectroscopic analysis as rutin. The amount of rutin in the leaves of Bn harvested from nine regions in South Korea (Bn 1-9) which were collected on the months of June, July, August, and September was determined by HPLC-UV analysis. A gradient elution program that utilizes a Discovery® C18 (4.6 × 250 mm, 5 μm) column and mobile phase composed of 1% acetic acid-water: acetonitrile (90:10 to 60:40 for min) was followed. The injection volume and flow rate were 10 μl and 1 mL/ min, respectively. UV detection was set at 350 nm. Results show that Bn-8 harvested in September reported the highest content of rutin among the samples analyzed. This study provides a basis for the optimal harvest time of Bn which maximizes the yield of rutin.
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KEYWORD
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Boehmeria nivea, HPLC-UV, Rutin, Content analysis
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