|
KMID : 0620720180240040288
|
|
Natural Product Sciences
2018 Volume.24 No. 4 p.288 ~ p.292
|
|
|
Efficient Isolation of Dihydrophaseic acid 3'-O-b-D-Glucopyranoside from Nelumbo nucifera Seeds Using High-performance Countercurrent Chromatography and Reverse-phased High-performance Liquid Chromatography
|
|
Rho Tae-Woong
Yoon Kee-Dong
|
|
|
Abstract
|
|
|
|
High-performance countercurrent chromatography (HPCCC) coupled with reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed to isolate dihydrophaseic acid 3'-O-b-D-glucopyranoside (DHPAG) from the extract of Nelumbo nucifera seeds. Enriched DHPAG sample (2.3 g) was separated by HPCCC using ethyl acetate/n-butanol/water system (6:4:10, v/v/v, normal-phase mode, flow rate: 4.0 mL/min) to give 23.1 mg of DHPAG with purity of 88.7%. Further preparative RP-HPLC experiment gave pure DHPAG (16.3 mg, purity > 98%). The current study demonstrates that utilization of CCC method maximizes the isolation efficiency compared with that of solid-based conventional column chromatography.
|
|
|
KEYWORD
|
|
|
Nelumbo nucifera, Dihydrophaseic acid 3'-O-β-D-glucopyranoside, High-performance countercurrent chromatography, Isolation efficiency
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|