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KMID : 0620720190250020111
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Natural Product Sciences
2019 Volume.25 No. 2 p.111 ~ p.114
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Simultaneous Analysis of Four Standards of The Herbal Formula, DF-02, of Ephedra intermedia and Rheum palmatum, using by High Performance Liquid Chromatography-Ultraviolet Detector (HPLC-UVD)
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Choi Seong-Yeon
Jeong Bi-Rang
Jang Hyeon-Seok
Lee Ji-Ho
Kwon Yong-Soo
Yoon Yoo-Sik
Shin Soon-Shik
Yang Hee-Jung
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Abstract
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The herbal formula, DF-02, consisting of Ephedra intermedia and Rheum palmatum are used for the treatment of the metabolic diseases such as obesity and liver fibrosis in Korean local clinics. We aimed to develop the simultaneous analytical conditions for four standards, (+)-pseudoephedrine (PSEP) and (-)-ephedrine (EP) for E. intermedia, and aloe-emodin (AE) and chrysophanol (CP) for R. palmatum using HPLC-UV techniques. The validated conditions yielded the high precision (relative standard deviation (RSD) < 3.65%) and the recoveries (94 - 106%) using the calibration curves with high linearity (R2 > 0.9994). As a result, four standards of DF-02 were simultaneously determined under the developed method, which will be utilized for the quality control or evaluation of DF-02 and many herbal preparations containing E. intermedia and R. palmatum.
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KEYWORD
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Ephedra intermedia, Rheum palmatum, (+)-pseudoephedrine, (-)-ephedrine, aloe-emodin, chrysophanol, HPLC, validation
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