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KMID : 0620720190250020122
Natural Product Sciences
2019 Volume.25 No. 2 p.122 ~ p.129
Quantitation and Radical Scavenging Activity Evaluation of Iridoids and Phenylethanoids from the Roots of Phlomis umbrosa (Turcz.) using DPPH Free Radical and DPPH-HPLC Methods, and their Cytotoxicity
Le Duc Dat

Nguyen Duc Hung
Zhao Bing Tian
Min Byung-Sun
Song Si-Whan
Woo Mi-Hee
Abstract
The roots of Phlomis umbrosa (Turcz.) (Phlomidis Radix) have been traditionally used to treat cold, reduce swelling and staunch bleeding. Four iridoids (1 - 3 and 5) and six phenylethanoid derivatives (4, and 6 - 10) were isolated from the roots of P. umbrosa. A simple, sensitive, and reliable analytical HPLC/PDA method was developed, validated, and applied to determine 10 marker compounds in Phlomidis Radix. Furthermore, the isolates were evaluated for cytotoxic and anti-oxidant activities as well as DPPH-HPLC method. Among them, compounds 4 and 6 - 9 displayed potent anti-oxidant capacities using DPPH assay with IC50 values of 27.7 ¡¾ 2.4, 10.2 ¡¾ 1.1, 18.0 ¡¾ 0.8, 19.1 ¡¾ 0.3, and 19.9 ¡¾ 0.6 ¥ìM, and compounds 6, 8, and 9 displayed significant cytotoxic activity against HL-60 with IC50 values of 35.4 ¡¾ 3.1, 18.6 ¡¾ 2.0, and 42.9 ¡¾ 3.0 ¥ìM, respectively.
KEYWORD
Phlomis umbrosa, antioxidant, developed HPLC/PDA method, cytotoxicity
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