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KMID : 0620720190250020150
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Natural Product Sciences
2019 Volume.25 No. 2 p.150 ~ p.156
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Simultaneous Extraction and Separation of Oil and Azadirachtin from Seeds and Leaves of Azadirachta indica using Binary Solvent Extraction
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Subramanian Sheela
Salleh Aiza Syuhaniz
Bachmann Robert Thomas
Hossain Md. Sohrab
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Abstract
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Conventional extraction of oil and azadirachtin, a botanical insecticide, from Azadirachta indica involves defatting the seeds and leaves using hexane followed by azadirachtin extraction with a polar solvent. In order to simplify the process while maintaining the yield we explored a binary extraction approach using Soxhlet extraction device and hexane and ethanol as non-polar and polar solvents at various ratios and extraction times. The highest oil and azadirachtin yields were obtained at 6 h extraction time using a 50:50 solvent mixture for both neem leaves (44.7 wt%, 720 mgAza/kgleaves) and seeds (53.5 wt%, 1045 mgAza/kgseeds), respectively.
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KEYWORD
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Azadirachtin, limonoids, neem oil, binary solvent extraction, botanical insecticide
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