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KMID : 0620720190250040317
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Natural Product Sciences
2019 Volume.25 No. 4 p.317 ~ p.325
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Anti-inflammatory and Immunosuppressive Effects of Panax notoginseng
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Cao Thao Quyen
Han Jae-Hyuk
Lee Hyun-Su
Ha Manh Tuan
Woo Mi-Hee
Min Byung-Sun
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Abstract
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Here, we designed to examine the anti-inflammatory effects on RAW264.7 cells and the immunosuppressive effects by evaluating interleukin-2 (IL-2) production in Jurkat T cells using a MeOH extract of Panax notoginseng roots. The results showed that the MeOH extract inhibited the synthesis of nitric oxide (NO) in a dose-dependent manner (IC50 value of 7.08 μg/mL) and displayed effects on T cell activation at a concentration of 400 μg/mL. In efforts to identify the potent compounds, bioactivity-guided fractionation of the MeOH extract and chemical investigation of its active CH2Cl2-, EtOAc-, and butanol- soluble fractions led to the successful isolation and identification of eleven compounds, including two polyacetylenes (1, 2), a steroid saponin (3), seven dammarane-type ginsenosides (4 - 10), and an oleanane-type ginsenoside (11). Among them, compound 11 was isolated from this plant for the first time. Compound 2 exhibited potent inhibitory effects on NO synthesis and an immunosuppressive effect with IC50 values of 2.28 and 65.57 μM, respectively.
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KEYWORD
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Panax notoginseng, anti-inflammation, immunosuppressive, ginsenoside, polyacetylene, saponin
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