KMID : 0620720220280040187
|
|
Natural Product Sciences 2022 Volume.28 No. 4 p.187 ~ p.193
|
|
Simultaneous Determination of Eight Compounds in Lysimachia christinae by HPLC-DAD
|
|
Ryu Ga-Hee
Ma Choong-Je
|
|
Abstract
|
|
|
Lysimachia christinae Hance was commonly used in Oriental medicine for treating the hepatitis virus, cholecystitis and cholagogic efficiency. According to the previous study, it possesses high antioxidant and anti-inflammatory activity. Simultaneous determination analytical method of isolated eight compounds, cynaroside (1), 2-(3,4-dimethoxyphenyl) ethyl O-¥á-L-arabinopyranosyl-(1¡æ2)-O-[6-deoxy-¥á-L-mannopyranosyl-(1¡æ3)] ¥â-D-glucopyranoside (2), stearylester ricinoleic acid (3), (E)-4-(3,4-dimethoxyphenyl) but-3-en-1-yl palmitate (4), 2-hydroxy-24-methoxy-4-tetracosenoic acid (5), 2-hydroxy-24-propoxy-4-tetracosenoic acid (6), ¥â-sitosterol (7), and androst-16-ene-3,6-diol (8) were established by using HPLC-DAD. This HPLC analysis was detected on a Dionex C18 column (5 ¥ìm, 120 A, 4.6 mm ¡¿ 150 mm) at 25¡É. The mobile phase consisted of 0.1% trifluoroacetic acid and acetonitrile at a flow rate of 1 mL/min. Validation of the method was assessed by linearity, precision and accuracy test. Calibration curve was good at r©÷ > 0.9998. Limits of detection (LOD) ranged from 0.19 to 8.18 g/ml and Limits of quantification (LOQ) ranged from 0.19 to 24.80 g/ml. The relative standard deviations (RSD) values of precision test, intra- and inter- day, were less than 0.99% and 1.0%. The accuracy test results ranged from 98.81% to 106.49% and RSD values were less than 0.95%. These results showed that the HPLC-DAD method was very reliable and accurate for the quantity analysis of eight compounds in L. christinae extract for quality control.
|
|
KEYWORD
|
|
Lysimachia christinae Hance, cynaroside, HPLC-DAD, quality control
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|