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KMID : 0620720230290040276
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Natural Product Sciences
2023 Volume.29 No. 4 p.276 ~ p.280
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Quantitative Analysis of Bioactive Compounds in a Mixture of the Lindera glauca Leaves Extract and Water-soluble Mastic Gum using an HPLC/UV Method
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Park Jin-Young
Jang Hyeon-Seok
Hong In-Kee
Yang Hee-Jung
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Abstract
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This study aimed to establish and validate an HPLC method for the quantitative analysis of bioactive compounds in a mixture of the L. glauca leaves extract and water-soluble mastic gum (MLM). MLM has shown potential as an effective agent for preventing hair loss in the previous study. For the development of the quality evaluation of MLM, quercitrin (1), isoquercitrin (2), and oleanonic acid (3) were selected as analytical markers. The separation was achieved using a reverse-phase column with a gradient solvent system of 0.1% formic acid aqueous-0.1% formic acid acetonitrile at a flow rate of 1.0 mL/min. Detection was carried out at 210 nm and 254 nm. The calibration curves for all three markers exhibited good linearity (R2 > 0.999). Recoveries of the three markers ranged from 100 ± 15%. The concentrations of compounds 1, 2, and 3 in MLM was determined to be 25.73 ± 1.38, 8.36 ± 0.05, and 212.24 ± 12.88 μg/mL, respectively. The validated method will facilitate further compositional investigations in MLM.
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KEYWORD
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Lindera glauca leaves, water-soluble mastic gum, HPLC method validation, quercitrin, isoquercitrin, oleanonic acid
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