KMID : 0620920000320040187
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Experimental & Molecular Medicine 2000 Volume.32 No. 4 p.187 ~ p.192
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Characterization of an unusual variant mRNA of human lysosomal ¥á-mannosidase
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John H Wolfe/Huaichang Sun
John H Wolfe
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Abstract
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Lysosomal ¥á-mannosidase (EC 3.2.1.24) is an exoglycosidase in the glycoprotein degradation pathway and is encoded by a 3.0 kb cDNA. A 2.3 kb cDNA from a minor species of HeLa cell mRNA was discovered by RT-PCR cloning. Southern blotting and PCR analysis of the HeLa cell genomic DNA showed that the 2.3 kb message was encoded by the lysosomal ¥á-mannosidase gene. Sequence comparison of the cDNA with the corresponding genomic DNA indicated that the 2.3 kb message was generated by an unusual intra-exonic joining event.
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KEYWORD
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lysosomal ¥á-mannosidase, intron sequences, mRNA, splice signal sequence,
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