KMID : 0620920210530091366
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Experimental & Molecular Medicine 2021 Volume.53 No. 9 p.1366 ~ p.1378
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MRTF-A-NF-¥êB/p65 axis-mediated PDL1 transcription and expression contributes to immune evasion of non-small-cell lung cancer via TGF-¥â
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Du Fu
Qi Xin Zhang Aotong Sui Fanfan Wang Xuemin Proud Christopher G. Lin Cunzhi Fan Xinglong Li Jing
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Abstract
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PD-L1 is abnormally regulated in many cancers and is critical for immune escape. Fully understanding the regulation of PD-L1 expression is vital for improving the clinical efficacy of relevant anticancer agents. TGF-¥â plays an important role in the low reactivity of PD-1/PD-L1 antibody immunotherapy. However, it is not very clear whether and how TGF-¥â affects PD-L1 expression. In the present study, we show that TGF-¥â upregulates the expression of the transcriptional coactivator MRTF-A in non-small-cell lung cancer cells, which subsequently interacts with NF-¥êB/p65 rather than SRF to facilitate the binding of NF-¥êB/p65 to the PDL1 promoter, thereby activating the transcription and expression of PD-L1. This leads to the immune escape of NSCLC cells. This process is dependent on the activation of the TGF-¥â signaling pathway. In vivo, inhibition of MRTF-A effectively suppresses the growth of lung tumor syngrafts with enrichment of NK and T cells in tumor tissue. Our study defines a new signaling pathway that regulates the transcription and expression of PD-L1 upon TGF-¥â treatment, which may have a significant impact on research into the application of immunotherapy in treating lung cancer.
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KEYWORD
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Non-small-cell lung cancer, Transcriptional regulatory elements
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