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KMID : 0624620190520120695
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BMB Reports
2019 Volume.52 No. 12 p.695 ~ p.699
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Transduced Tat-CIAPIN1 reduces the inflammatory response on LPS- and TPA-induced damages
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Yeo Hyeon-Ji
Shin Min-Jea
You Ji-Ho
Kim Jeong-Su
Kim Min-Young
Kim Dae-Won
Kim Duk-Soo
Eum Won-Sik
Choi Soo-Young
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Abstract
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Cytokine-induced apoptosis inhibitor 1 (CIAPIN1), known as an anti-apoptotic and signal-transduction protein, plays a pivotal role in a variety of biological processes. However, the role of CIAPIN1 in inflammation is unclear. We investigated the protective effects of CIAPIN1 in lipopolysaccharide (LPS)-exposed Raw 264.7 cells and against inflammatory damage induced by 12-O-tetradecanoylphorbol-13-acetate (TPA) in a mouse model using cell-permeable Tat-CIAPIN1. Transduced Tat-CIAPIN1 significantly reduced ROS production and DNA fragmentation in LPS-exposed Raw 264.7 cells. Also, Tat-CIAPIN1 inhibited MAPKs and NF-κB activation, reduced the expression of Bax, and cleaved caspase-3, COX-2, iNOS, IL-6, and TNF-α in LPS-exposed cells. In a TPA-induced animal model, transduced Tat-CIAPIN1 drastically decreased inflammation damage and inhibited COX-2, iNOS, IL-6, and TNF-α expression. Therefore, these findings suggest that Tat-CIAPIN1 might lead to a new strategy for the treatment of inflammatory skin disorders.
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KEYWORD
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Cytokine, Inflammation, MAPK, Protein therapy, Tat-CIAPIN1
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