KMID : 0665220200330030309
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Korean Journal of Food and Nutrition 2020 Volume.33 No. 3 p.309 ~ p.316
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Protective Effects of Ulva lactuca Methanol Extracts against the Ultraviolet B-induced DNA Damage
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Jeong Seul-A
Chung Yu-Heon Park Jong-Kun
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Abstract
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In this study, we investigated the protective effects of Ulva lactuca methanol extracts against ultraviolet B (UVB)-induced DNA damage in HaCaT cells. First, the contents of general and antioxidative nutrient contents of Ulva lactuca were measured. The moisture, carbohydrate, crude protein, crude fat and ash were 14.01%, 44.80%, 23.19%, 3.10% and 14.90%, respectively. Magnesium that acts as DNA repair enzyme cofactor was the most abundant mineral followed by Ca, P and Fe. The total phenolic and anthocyanoside contents of Ulva lactuca were 2.69 mg/g and 0.13 mg/g, respectively. Cells treated with Ulva lactuca methanol extracts for 24 hours post UVB exposure increased cell viability in a concentration-dependent manner compared to the non-treated control. Also, Ulva lactuca methanol extracts decreased the levels of UVB-induced DNA damage such as cyclobutane pyrimidine dimer and DNA damage response (DDR) proteins such as p-p53 and p21. These results suggest that Ulva lactuca methanol extracts comprising physiological active substances such as Mg, polyphenols and anthocyanosides promote DNA repair by regulating genes related with DDR. Key words: Ulva lactuca, ultraviolet B, DNA damage
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KEYWORD
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Ulva lactuca, ultraviolet B, DNA damage
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