KMID : 0811720160200040407
|
|
Korean Journal of Physiology & Pharmacology 2016 Volume.20 No. 4 p.407 ~ p.414
|
|
Involvement of spinal muscarinic and serotonergic receptors in the anti-allodynic effect of electroacupuncture in rats with oxaliplatin-induced neuropathic pain
|
|
Lee Ji-Hwan
Go Dong-hyun Kim Woo-jin Lee Gi-seog Bae Hyo-jeong Fu Shi Quan Kim Sun-Kwang
|
|
Abstract
|
|
|
This study was performed to investigate whether the spinal cho-linergic and serotonergic analgesic systems mediate the relieving effect of elec-troacupuncture (EA) on oxaliplatin-induced neuropathic cold allodynia in rats. The cold allodynia induced by an oxaliplatin injection (6 mg/kg, i.p.) was evaluated by immersing the rat¡¯s tail into cold water (4oC) and measuring the withdrawal latency. EA stimulation (2 Hz, 0.3-ms pulse duration, 0.2~0.3 mA) at the acupoint ST36, GV3, or LI11 all showed a significant anti-allodynic effect, which was stronger at ST36. The analgesic effect of EA at ST36 was blocked by intraperitoneal injection of muscarinic acetylcholine receptor antagonist (atropine, 1 mg/kg), but not by nicotinic (mecamylamine, 2 mg/kg) receptor antagonist. Furthermore, intrathecal administration of M2(methoctramine, 10¥ìg) and M3 (4-DAMP, 10¥ìg) receptor antagonist, but not M1 (pirenzepine, 10¥ìg) receptor antagonist, blocked the effect. Also, spinal administration of 5-HT3 (MDL-72222, 12¥ìg) receptor antagonist, but not 5-HT1A (NAN-190, 15¥ìg) or 5-HT2A (ketanserin, 30¥ìg) receptor antagonist, prevented the anti-allodynic effect of EA. These results suggest that EA may have a signi? cant analgesic action against oxaliplatin-induced neuropathic pain, which is mediated by spinal cholinergic (M2, M3) and serotonergic (5-HT3) receptors.
|
|
KEYWORD
|
|
Acetylcholine, Cold allodynia, Electroacupuncture, Oxaliplatin, Serotonin
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|