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KMID : 0848020020050020154
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Journal of Korean Breast Cancer Society
2002 Volume.5 No. 2 p.154 ~ p.160
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Detection of Axillary Lymph Node Micrometastases in Breast Cancer Using RT-PCR Comparison the Results of MUC1, Cytokeratin 19
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이령아/Ryung Ah Lee
강희준/김성원/강한성/김성숙/윤여규/오승근/최국진/노동영/Hee Joon Kang/Sung Won Kim/Han Sung Kang/Seong Suk Kim/Yeo Kyu Yun/Seung Keun Oh/Kuk Jin Choe/Dong Young Noh
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Abstract
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Purpose: The detection of axillary lymph-node micrometastases in breast-cancer patients by using the reverse transcriptase-polymerase chain reaction (RT-PCR) may provide a good guide for postoperative therapy. To evaluate effectiveness of
RT-PCR
for the detection of micrometastasis, we have compared the results of conventional, immunohistochemical (IHC) staining with those of RT-PCR.
Methods: We conducted RT-PCR amplifications of MUC1 and cytokeratin (CK) 19 on lymph nodes from 40 breast-cancer patients and conducted IHC staining for cytokeratin. The results of histological examination and cytokeratin IHC staining were
compared with the RT-PCR results for the detection of lymph-node micrometastases.
Results: Nineteen of 40 cases (47.5%) were lymph-node positive and twenty-one cases (52.5%) were lymph-node negative. Using RT-PCR MUC1 and CK19 expressions were detected in all positive lymph nodes and in 4 (19.0%) and 5 (23.8%), in
negative
nodes, respectively. Not all positive nodes were stained by IHC (94.7%), and none of the negative lymph nodes were stained by IHC. Thus, IHC staining was ineffective in detecting micrometastases.
Conclusion: Detection of MUC1 and CK19 by using RT- PCR can be a more accurate and useful method than IHC staining for the detection of axillary lymph-node micrometastases in breast cancer.
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KEYWORD
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