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KMID : 0880420140150040448
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Korean Journal of Radiology
2014 Volume.15 No. 4 p.448 ~ p.455
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Serial Micro-CT Assessment of the Therapeutic Effects of Rosiglitazone in a Bleomycin-Induced Lung Fibrosis Mouse Model
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Choi Eun-Jung
Jin Gong-Yong
Bok Se-Mi
Han Young-Min
Lee Young-Sun
Jung Myung-Ja
Kwon Keun-Sang
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Abstract
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Objective: The aim of this study was to assess the therapeutic effects of rosiglitazone with serial micro-CT findings before and after rosiglitazone administration in a lung fibrosis mouse model induced with bleomycin.
Materials and Methods: We instilled the bleomycin solution directly into the trachea in twenty mice (female, C57BL/6 mice). After the instillation with bleomycin, mice were closely observed for 3 weeks and then all mice were scanned using micro-CT without sacrifice. At 3 weeks, the mice were treated with rosiglitazone on days 21 to 27 if they had abnormal CT findings (n = 9, 45%). For the mice treated with rosiglitazone, we performed micro-CT with mouse sacrifice 2 weeks after the rosiglitazone treatment completion. We assessed the abnormal CT findings (ground glass attenuation, consolidation, bronchiectasis, reticular opacity, and honeycombing) using a five-point scale at 3 and 6 weeks using Wilcoxon-signed ranked test. The micro-CT findings were correlated with the histopathologic results.
Results: One out of nine (11.1%) mice improved completely. In terms of consolidation, all mice (100%) showed marked decrease from 3.1 ± 1.4 at 3 weeks to 0.9 ± 0.9 at 6 weeks (p = 0.006). At 6 weeks, mild bronchiectasis (n = 6, 66.7%), mild reticular opacity (n = 7, 77.8%) and mild honeycomb patterns (n = 3, 33.3%) appeared.
Conclusion: A serial micro-CT enables the evaluation of drug effects in a lung fibrosis mouse model.
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KEYWORD
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Microcomputed tomography, Bleomycin, Fibrosis, Rosiglitazone, Lung
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