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KMID : 0893320030180040305
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Journal of Environmental Toxicology
2003 Volume.18 No. 4 p.305 ~ p.310
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Heave Metal Toxicity Test in Moina macrocopa with Glucose-6-phosphate Dehydrogenase Activity
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Park Yong-Seok
Lee Sang-Goo
Lee Seung-Jin
Moon Sung-Kyung
Choi Eun-Joo
Rhie Ki-Tae
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Abstract
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A rapid, inexpensive enzymatic method is proposed for indirect water quality testing in terms of heavy metal toxicity. The activity of glucose-6-phosphate dehydrogenase was applied for heavy metal toxicity test as an effective criterion in water quality. The toxicity of Pb (lead) and Cd (cadmium) for water flea, Moina macrocopa, were evaluated for 2-8 days with variables of mobilization ability. And the reproduction impairment of Moina macrocopa were investigated as the parameter of chronic toxicity test for Pb and Cd. As a result, the EC for immobilization of Moina macrocopa were Pb and Cd were 1.6749 and 0.4683, respectively. The values of reproductive impairment to Moina macrocopa for Pb and Cd were 9.5938 and 8.3264 in EC A significant alteration of G6PDH (Glucose-6-phosphate dehydrogenase) activity of Moina macrocopa was observed when Cd and Pb were treated in media. The results obtained indicate that G6PDH activity of Moina macrocopa can be used as an indicative parameter in aquatic toxicity tests for heavy metals.als.
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KEYWORD
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Cd, Pb, toxicity, Moina macrocopa, glucose -6 phosphate dehydrogenase
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