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KMID : 0893320050200020187
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Journal of Environmental Toxicology
2005 Volume.20 No. 2 p.187 ~ p.194
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Molecular Biomarkers of Octachlorostyrene Exposure in Medaka, Oryzias latipes, using Microarray Technique
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You Dae-Eun
Kang Mi-Sun
Park Eun-Jeung
Kim Il-Chan
Lee Jae-Seong
Park Kwang-Sik
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Abstract
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Octachlorostyrene (OCS) is a primarily concerning chemical in many countries because of its persistent and bioaccumulative properties in the environment. OCS is not commercially manufactured or used but it may be produced during incineration or chemical synthetic processes involving chlorinated compounds. There are several reports that OCS was found in the waters, sediments, fish, mussels, and also in human tissues. However, systematic studies on the OCS toxicities are scarce in literature. In this study, we tried to get the gene expression data using medaka DNA chip to identify biomarkers of OCS exposure. Medaka (Oryzias latipes.) was exposed to OCS 1 ppm for 2 days and 10 days, respectively. Total RNA was extracted and purified by guanidine thiocyanate method and the Cy3- and Cy5-labelled cDNAs produced by reverse trancription of the RNA were hybridized to medaka microarray. As results, eighty five genes were found to be down-or up regulated by OCS. Some of the genes were listed and confirmed by real-time PCR.
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KEYWORD
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octachlorostyrene, biomarker, medaka (Oryzias latipes), microarray
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Listed journal information
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