|
KMID : 0893320100250020111
|
|
Journal of Environmental Toxicology
2010 Volume.25 No. 2 p.111 ~ p.120
|
|
|
Cytotoxicity of Diesel Exhaust Particles from Various Vehicles toward Macrophage Cells
|
|
Lee Jang-Han
Lee Yong-Kwon
Lee Ji-Young
Lee Seung-Bok
Kim Sun-Hwa
Bae Gwi-Nam
Lee Hak-Sung
Lim Cheol-Soo
Chung Nam-Hyun
|
|
|
Abstract
|
|
|
|
DEPs (diesel exhaust particles) like any other particles can be also inhaled into lung to participate in a damaging reaction to the organ. Possible damages might be apoptosis and inflammatory responses to the cells in respiratory track. The aim of this study was cytotoxicity evaluation of DEPs from five in-use diesel vehicles using a murine macrophage cell (RAW 254.7). We found that most DEPs have a considerable cytotoxicity compared to the control and SRM 2975. When measured by MIT assay and extents of apoptosis, DEPs of two high-mileage vehicles had higher toxicity than those of the other three low-mileage vehicles tested. Although mRNA expression level of TNF-α. somewhat explains the trend of cytotoxicity and apoptosis, that of IL-1β did not. Correlation studies among the extents of MIT assay, apoptosis, and TNF-α. expression showed that the extents between apoptosis and TNF-α expression was most highly correlated (r=0.96). These results suggest that cytotoxicity of various DEPs could be compared easily by measuring the extent of apoptosis or TNF-α expression by DEPs.
|
|
|
KEYWORD
|
|
|
Diesel exhaust particle, Macrophage, Cytotoxicity, Apoptosis, TNF-α expression
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|