|
KMID : 0893420060070040369
|
|
Journal of Veterinary Science
2006 Volume.7 No. 4 p.369 ~ p.374
|
|
|
Detection of betanodaviruses in apparently healthy aquarium fishes and invertebrates
|
|
Dennis Kaw Gomez
Lim Dong Joo
Baeck Gun Wook
Youn Hee Jeong
Shin Nam Shik
Youn Hwa Young
Park Jun Hong
Park Se Chang
|
|
|
Abstract
|
|
|
|
Betanodaviruses are the causative agents of viral nervous necrosis (VNN) in cultured marine fish. A total of 237 apparently healthy aquarium fish, marine (65 species) and freshwater (12 species) fishes and marine invertebrates (4 species), which were stocked in a commercial aquarium in Seoul, South Korea, were collected from November 2005 to February 2006. The brains of the fish and other tissues of the invertebrates were examined by reverse transcriptasepolymerase chain reaction (RT-PCR) and nested PCR to detect betanodavirus. Positive nested PCR results were obtained from the brains of 8 marine fish species (shrimp fish Aeoliscus strigatus, milkfish Chanos chanos, three spot damsel Dascyllus trimaculatus, Japanese anchovy Engraulis japonicus, pinecone fish Monocentris japonica, blue ribbon eel Rhinomuraena quaesita, look down fish Selene vomer, yellow tang Zebrasoma flavesenes), 1 marine invertebrate species (spiny lobster Pamulirus versicolor), and 2 freshwater fish species (South American leaf fish Monocirrhus polyacanthus and red piranha Pygocentrus nattereri). The detection rate in nested PCR was 11/237 (4.64%). These subclinically infected aquarium fish and invertebrates may constitute an inoculum source of betanodaviruses for cultured fishes in the Korean Peninsula.
|
|
|
KEYWORD
|
|
|
aquarium fish, betanodavirus, invertebrate, PCR detection, subclinical infection, viral nervous necrosis
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
   
|
|