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KMID : 0893420070080020175
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Journal of Veterinary Science
2007 Volume.8 No. 2 p.175 ~ p.180
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Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus
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Amr E. El Hakim
Yasser E. Shahein
Amira M. K. Abouelella
Mohamed E. Selim
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Abstract
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The present study was conducted to identify new target immunogenic molecules from the larval stage of the cattle tick, Boophilus annulatus (Acari: Ixodidae). Two specific larval glycoproteins (GLPs) were isolated by two-step affinity chromatography. The larval immunogens were first purified with CNBr-Sepharose coupled to rabbit anti-larval immunoglobulins, and the glycoproteins were then purified with Con-A Sepharose. These glycoproteins have molecular weights of approximately 32 and 15 kDa with isoelectric points between 6.8 and 7.2. Antibodies against the two GLPs, labeled I and II, were detected in the anti-whole tick, -whole larval, and -gut antigens through immunoblot analysis. These results suggest that these GLPs are good immunogens and can be useful in the vaccination of cattle against tick infestation.
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KEYWORD
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Boophilus annulatus, glycoproteins, larvae, vaccination.
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