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KMID : 0893420080090040423
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Journal of Veterinary Science
2008 Volume.9 No. 4 p.423 ~ p.425
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Circadian variations in salivary chromogranin a concentrations during a 24-hour period in dogs
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Kanai Kazutaka
Hoshi Fumio
Itoh Naoyuki
Higuchi Seiichi
Hino Mariko
Hori Yasutomo
Nakao Ruriko
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Abstract
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The purpose of this study was to determine if salivary chromogranin a secretion in dogs exhibits a circadian rhythm. Saliva sampling was performed during three different sessions occurring in three nonconsecutive 24-h periods. Sixteen healthy adult beagle dogs (8 males and 8 females) were moved to a sampling room and housed individually in cages. Saliva samples were obtained every 4 h from 12:00 p.m. to 12:00 p.m. the following day. In the interest of habituation, saliva was obtained hourly from each dog 3 h before the experiment was started. Salivary chromogranin A concentrations were measured using an enzyme-linked immunosorbent assay. No circadian rhythm was detected for salivary chromogranin A secretion, and no differences in salivary chromogranin A concentrations measured every 4 h were demonstrated during the 24-h cycle in dogs.
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KEYWORD
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chromogranin A, circadian variations, dog, saliva
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