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KMID : 0893420090100020121
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Journal of Veterinary Science
2009 Volume.10 No. 2 p.121 ~ p.130
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Genetic analysis of ORF5 of recent Korean porcine reproductive and respiratory syndrome viruses (PRRSVs) in viremic sera collected from MLV-vaccinating or non-vaccinating farms
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Kim Hye-Kwon
Park Bong-Kyun
Park Seong-Jun
Lee Chul-Seung
Luo Yuzi
Moon Hyoung-Joon
Yang Jeong-Sun
Song Dae-Sub
Kang Bo-Kyu
Ann Soo-Kyung
Jun Chan-Hyuk
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Abstract
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The 23 open reading frame (ORF) 5 sequences of Korean type II porcine reproductive and respiratory syndrome virus (PRRSV) were collected from viremic sera from the (modified live vaccine) MLV-vaccinating and non-vaccinating farms from 2007 to 2008. The samples were phylogenetically analyzed with previous ORF5 sequences, including type I Korean PRRSV, and previously reported or collected sequences from 1997 to 2008. A MN184-like subgroup of type II Korean PRRSV was newly identified in the viremic sera collected from 2007 to 2008. And of the type I PRRSVs, one subgroup had 87.2∼88.9% similarity with the Lelystad virus, showing a close relationship with the 27∼2003 strain of Spain. The maximum parsimony tree of type II PRRSV from 1997 to 2008 showed that they had evolved to four lineages, subgroups 1, 2, 3 and 4. Most of the recently collected type II PRRSVs belonged to subgroup 4 (48%). The region of three B-cell epitopes and two T-cell epitopes of ORF5 amino acids sequences was considerably different from the MLV in subgroups 3 and 4. In conclusion, the existence of type I PRRSV, which was genetically different from Lelystad virus (Prototype of type I PRRSV), and heterologous type II PRRSVs of viremic pigs detected even in the MLV-vaccinating farms indicated the need for new vaccine approaches for the control of PRRSV in Korea
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KEYWORD
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ORF5, phylogenetic, PRRSV, type, viremia
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