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KMID : 0893420090100030197
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Journal of Veterinary Science
2009 Volume.10 No. 3 p.197 ~ p.201
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Role of protease inhibitors and acylation stimulating protein in the adipogenesis in 3T3-L1 cells
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Mohamed Mohamed Soliman
Yakut Abdel-Fattah El-Senosi
Maysara Mahmoud Salem
Omniya Mahmoud Abdel Hamid
Kimura Kazuhiro
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Abstract
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Treatment of AIDS (HIV) and hepatitis C virus needs protease inhibitors (PI) to prevent viral replication. Uses of PI in therapy are usually associated with a decrease in body weight and dyslipidemia. Acylation stimulating protein (ASP) is a protein synthesized in adipocytes to increase triglycerides biosynthesis, for that the relation of PI and ASP to adipogenesis is tested in this work. ASP expression was increased during 3T3-L1 differentiation and reached a peak at day 8 with cell maturation. Addition of PI during adipocytes differentiation dose dependently and significantly (p ＜ 0.5) inhibited the degree of triglycerides (TG) accumulation. Moreover, presence of ASP (450 ng/mL) in media significantly (p ＜ 0.5) stimulated the degree of TG accumulation and there was additive stimulation for ASP when added with insulin (10 μg/mL). Finally, when ASP in different doses (Low, 16.7; Medium, 45 and High, 450 ng/mL) incubated with a dose of ×150 PI, ASP partially inhibited the PI-inhibited adipogenesis and TG accumulation. The results in this study show that PI inhibit lipids accumulation and confirm role of ASP in TG biosynthesis and adipogenesis.
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KEYWORD
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acylation stimulating protein, adipogenesis, 3T3L-1 cells
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