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KMID : 0893420090100030261
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Journal of Veterinary Science
2009 Volume.10 No. 3 p.261 ~ p.263
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Isolation and identification of a canine coronavirus strain from giant pandas (Ailuropoda melanoleuca)
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Gao Feng-Shan
Hu Gui-Xue
Xia Xian-zhu
Gao Yu-Wei
Bai Ya-Duo
Zou Xiao-Huan
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Abstract
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Two giant pandas (Ailuropoda melanoleuca) died of unknown causes in a Chinese zoo. The clinical disease profile suggested that the pandas may have suffered a viral infection. Therefore, a series of detection including virus isolation, electron microscopy, cytobiological assay, serum neutralization and RT-PCR were used to identify the virus. It was determined that the isolated virus was a canine coronavirus (CCV), on the basis of coronavirus, neutralization by canine anti-CCV serum, and 84.3% to 100% amino acid sequence similarity with CCV. The results suggest that the affected pandas had been infected with CCV.
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KEYWORD
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canine coronavirus, giant panda, virus characterization, virus isolation
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