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KMID : 0893420100110010085
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Journal of Veterinary Science
2010 Volume.11 No. 1 p.85 ~ p.87
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Plasmid profiling of Flavobacterium psychrophilum isolates from ayu (Plecoglossus altivelis altivelis) and other fish species in Japan
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Kim Ji-Hyung
Dennis K. Gomez
Nakai Toshihiro
Park Se-Chang
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Abstract
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In order to evaluate the genetic variability of the causative agent of cold water disease (CWD), plasmid profiling was used to characterize Flavobacterium (F.) psychrophilum isolates (n = 169). Size analysis of plasmids in F. psychrophilum isolates (n = 128) from several fish species demonstrated that six kinds of plasmids were harbored, and ayu isolates had different profiles compared to other isolates. Moreover, multiple isolates (n = 41) from CWD outbreaks in 2002 to 2003 at a single ayu farm were examined to determine differences between isolates from successive outbreaks and showed different profiles by the sources of seedlings.
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KEYWORD
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ayu, CWD, Flavobacterium psychrophilum, plasmid profile
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