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KMID : 0893420100110020165
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Journal of Veterinary Science
2010 Volume.11 No. 2 p.165 ~ p.167
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Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43
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Lee Hyun-Kyoung
Lee Byoung-Hee
Seok Seung-Hyeok
Baek Min-Won
Lee Hui-Young
Kim Dong-Jae
Na Yi-Rang
Noh Kyoung-Jin
Park Sung-Hoon
Dutta Noton Kumar
Kariwa Hiroaki
Nakauchi Mina
Heo Suk-Jin
Park Jae-Hak
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Abstract
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Severe acute respiratory syndrome (SARS) is a life-threatening disease for which accurate diagnosis is essential. Although many tools have been developed for the diagnosis of SARS, false-positive reactions in negative sera may occur because of cross-reactivity with other coronaviruses. We have raised polyclonal and monoclonal antibodies (Abs) using a recombinant form of the SARS virus nucleocapsid protein. Cross-reactivity of these anti-SARS Abs against human coronavirus (HCoV) 229E and HCoV OC43 were determined by Western blotting. The Abs produced reacted with recombinant SARS virus nucleocapsid protein, but not with HCoV 229E or HCoV OC43.
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KEYWORD
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cross-reactivity, HCoV 229E, HCoV OC43, recombinant nucleocapsid protein, SARS
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