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KMID : 0893420100110040327
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Journal of Veterinary Science
2010 Volume.11 No. 4 p.327 ~ p.332
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Optimization of culture media of pathogenic Mycoplasma hyopneumoniae by a response surface methodology
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Hwang Mi-Hyun
Damte Dereje
Cho Min-Hee
Kim Young-Hoan
Park Seung-Chun
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Abstract
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Composition of culture medium for mass production of Mycoplasma hyopneumoniae was optimized using a response surface methodology (RSM). Initially, the influence of glucose, thallium acetate, fresh yeast extract, horse serum, and porcine serum on the production of mycoplasmal protein was assessed using a ’one factor at a time’ technique. Next, factors with a significant effect, including fresh yeast extract, and horse and porcine sera, were selected for further optimization using a central composite design (CCD) of RSM. The experimental results were fitted into a second order polynomial model equation. Estimated optimal condition of the factors for maximum production of mycoplasmal protein (i.e., triple-fold increase from 0.8 mg/L produced by basal mycoplasma media to 2.5 mg/L) was 10.9% fresh yeast extract, 15% horse serum, and 31.5% porcine serum (v/v). For the optimized conditions, a 2.96 mg/L experimental result was observed, similar to the estimated optimal conditions result of the CCD.
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KEYWORD
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fresh yeast extract, horse serum, Mycoplasma hyopneumoniae, optimization, response surface analysis
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