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KMID : 0893420100110040355
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Journal of Veterinary Science
2010 Volume.11 No. 4 p.355 ~ p.357
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Development of a universal plate-agglutination test for detecting Haemophilus parasuis
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Guo Dingqian
Tang Cheng
Hai Quan
Shao Guoqing
Yue Hua
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Abstract
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Due to the serovar diversity in Haemophilus (H.) parasuis, it is difficult to develop a universal serological method for detection of this pathogen. Here, we report a universal plate-agglutination test for detecting H. parasuis. Diagnostic antisera were prepared by mixing antisera of serovars 4, 5, 12, 13 and 14 in the optimized ratio. The results of the plate-agglutination test showed that the diagnostic antisera could agglutinate with all 15 reference strains of H. parasuis and 74/75 clinical isolates. Further, the specificity of the method was validated with 22 bacterial strains from 12 related species.
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KEYWORD
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diagnostic antisera, Haemophilus parasuis, plate-agglutination
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