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KMID : 0893420110120010041
Journal of Veterinary Science
2011 Volume.12 No. 1 p.41 ~ p.48
Molecular characterization of tetracycline- and quinolone-resistant Aeromonas salmonicida isolated in Korea
Kim Ji-Hyung

Hwang Sun-Young
Son Jee-Soo
Han Jee-Eun
Jun Jin-Woo
Shin Sang-Phil
Jr Casiano Choresca
Choi Yun-Jaie
Park Yong-Ho
Park Se-Chang
Abstract
The antibiotic resistance of 16 Aeromonas (A.) salmonicida strains isolated from diseased fish and environmental samples in Korea from 2006 to 2009 were investigated in this study. Tetracycline or quinolone resistance was observed in eight and 16 of the isolates, respectively, based on the measured minimal inhibitory concentrations. Among the tetracycline-resistant strains, seven of the isolates harbored tetA gene and one isolate harbored tetE gene. Additionally, quinolone-resistance determining regions (QRDRs) consisting of the gyrA and parC genes were amplified and sequenced. Among the quinolone-resistant A. salmonicida strains, 15 harbored point mutations in the gyrA codon 83 which were responsible for the corresponding amino acid substitutions of Ser83¡æArg83 or Ser83¡æAsn83. We detected no point mutations in other QRDRs, such as gyrA codons 87 and 92, and parC codons 80 and 84. Genetic similarity was assessed via pulsed-field gel electrophoresis, and the results indicated high clonality among the Korean antibiotic-resistant strains of A. salmonicida.
KEYWORD
Aeromonas salmonicida, minimal inhibitory concentration, pulsed-field gel electrophoresis, quinolone- resistance determining region, tetracycline-resistance
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