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KMID : 0893420110120010095
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Journal of Veterinary Science
2011 Volume.12 No. 1 p.95 ~ p.97
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Invasive potential of biofilm-forming Staphylococci bovine subclinical mastitis isolates
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Oliveira Manuela
Bexiga Ricardo
Nunes Sandro Filipe
Vilela Cristina Lobo
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Abstract
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Staphylococcus (S.) aureus is a common infectious agent of bovine chronic mastitis, a disease that is difficult to eradicate. The abilities of Staphylococci to be internalized and form a biofilm can contribute to host immunological defence evasion that subsequently impairs antimicrobial therapy. The invasive capability of six S. aureus field isolates with different biofilm- forming profiles was compared in vitro using a bovine mammary epithelial cell line. This was further confirmed in primary cell cultures using fluorescent rRNA probes against S. aureus. The results suggest that S. aureus invasion levels are not related to biofilm formation.
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KEYWORD
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biofilm, bovine mammary epithelial cells, bovine subclinical mastitis, invasion, Staphylococci
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