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KMID : 0893420110120030295
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Journal of Veterinary Science
2011 Volume.12 No. 3 p.295 ~ p.297
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Development of a novel diagnostic test for detection of bovine viral diarrhea persistently infected animals using hair
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Singh Kuldeep
Miller Myrna M.
Kohrt Laura J.
Scherba Gail
Garrett Edgar F.
Fredrickson Richard L.
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Abstract
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The purpose of this study was to determine whether manually plucked hairs might serve as an alternative sample for a quantitative real time polymerase chain reaction (qRT-PCR) testing. Twenty three, 1~3 week old, non-bovine viral diarrhea virus (BVDV) vaccinated calves, found to be positive for BVDV by immunohistochemical staining, were selected and hairs were manually plucked from the ear. qRT-PCR was performed on samples consisting of more than 30 hairs (30~100) and whole blood. All 23 animals were positive for the virus by qRT-PCR performed on the whole blood and when samples of more than 30 hairs were assayed. Additionally, qRT-PCR was performed on groups of 10 and 20 hairs harvested from 7 out of 23 immunohistochemical staining-positive calves. When groups of 20 and 10 hairs were tested, 6 and 4 animals, respectively, were positive for the virus.
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KEYWORD
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bovine viral diarrhea virus, diagnostic tests, hair, persistent infection, RT-PCR
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