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KMID : 0893420120130010043
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Journal of Veterinary Science
2012 Volume.13 No. 1 p.43 ~ p.48
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Comparison of four diagnostic methods for detecting rabies viruses circulating in Korea
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Yang Dong-Kun
Shin Eun-Kyung
Oh Yoon-I
Lee Kyung-Woo
Lee Chung-San
Kim Seo-Young
Lee Jeong-Ah
Song Jae-Young
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Abstract
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It is essential to rapidly and precisely diagnose rabies. In this study, we evaluated four diagnostic methods, indirect fluorescent antibody test (FAT), virus isolation (VI), reverse transcriptase polymerase chain reaction (RT-PCR), and rapid immunodiagnostic assay (RIDA), to detect rabies in animal brain homogenates. Out of the 110 animal brain samples tested, 20 (18.2%) were positive for rabies according to the FAT. Compared to the FAT, the sensitivities of VI, RT-PCR, and RIDA were 100, 100, and 95%, respectively. The specificities of VI, RT-PCR and RIDA were found to be 100, 100, and 98.9%, respectively. Rabies viruses circulating in Korea were isolated and propagated in murine neuroblastoma (NG108-15) cells with titers ranging from 101.5 to 104.5 TCID50/mL. Although the RIDA findings did not completely coincide with results obtained from FAT, VI, and RT-PCR, RIDA appears to be a fast and reliable assay that can be used to analyze brain samples. In summary, the results from our study showed that VI, RT-PCR, and RIDA can be used as supplementary diagnostic tools for detecting rabies viruses in both laboratory and field settings.
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KEYWORD
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immunodiagnostic assay, rabies, RT-PCR, virus isolation
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