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KMID : 0893420120130010059
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Journal of Veterinary Science
2012 Volume.13 No. 1 p.59 ~ p.65
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Induction of humoral responses to BHV-1 glycoprotein D expressed by HSV-1 amplicon vectors
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Blanc Andrea Maria
Beatriz Berois Mabel
Tome Lorena Magali
Epstein Alberto L.
Arbiza Juan Ramon
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Abstract
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Herpes simplex virus type-1 (HSV-1) amplicon vectors are versatile and useful tools for transferring genes into cells that are capable of stimulating a specific immune response to their expressed antigens. In this work, two HSV-1-derived amplicon vectors were generated. One of these expressed the full-length glycoprotein D (gD) of bovine herpesvirus 1 while the second expressed the truncated form of gD (gDtr) which lacked the trans-membrane region. After evaluating gD expression in the infected cells, the ability of both vectors to induce a specific gD immune response was tested in BALB/c mice that were intramuscularly immunized. Specific serum antibody responses were detected in mice inoculated with both vectors, and the response against truncated gD was higher than the response against full-length gD. These results reinforce previous findings that HSV-1 amplicon vectors can potentially deliver antigens to animals and highlight the prospective use of these vectors for treating infectious bovine rhinotracheitis disease.
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KEYWORD
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antibody response, BHV-1 gD, HSV-1 amplicon vectors, immunization
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