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KMID : 0893420120130040429
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Journal of Veterinary Science
2012 Volume.13 No. 4 p.429 ~ p.432
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Development of monoclonal antibodies against the abnormal prion protein isoform (PrPres) associated with chronic wasting disease (CWD)
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Jeong Hyun-Jeong
Lee Nak-Hyung
Lee Joong-Bok
Park Seung-Yong
Song Chang-Seon
Seo Kun-Ho
Kim Dong-Woon
Kim Yong-Sun
Choi In-Soo
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Abstract
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Monoclonal antibodies (mAbs) specific for the abnormal prion protein isoform (PrPres) are indispensable for diagnosing chronic wasting disease (CWD). In this study, eight mAbs were developed by immunizing PrP knockout mice with recombinant elk PrP and an immunogenic PrP peptide. The reactivity of the mAbs to recombinant PrP and the PrP peptide was measured, and their isotypes were subsequently determined. Among them, four mAbs (B85-05, B85-08, B85-12, and B77-75) were shown by Western blotting to recognize proteinase K-treated brain homogenate derived from an elk suffering from CWD.
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KEYWORD
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CWD, elk, monoclonal antibody, prion, PrPres
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