|
KMID : 0893420130140010069
|
|
Journal of Veterinary Science
2013 Volume.14 No. 1 p.69 ~ p.76
|
|
|
Survival of hypoxic human mesenchymal stem cells is enhanced by a positive feedback loop involving miR-210 and hypoxia-inducible factor 1
|
|
Chang Woo-Chul
Lee Chang-Youn
Park Jun-Hee
Park Moon-Seo
Maeng Lee-So
Yoon Chee-Soon
Lee Min-Young
Hwang Ki-Chul
Chung Yong-An
|
|
|
Abstract
|
|
|
|
The use of mesenchymal stem cells (MSCs) has emerged as a potential new treatment for myocardial infarction. However, the poor viability of MSCs after transplantation critically limits the efficacy of this new strategy. The expression of microRNA-210 (miR-210) is induced by hypoxia and is important for cell survival under hypoxic conditions. Hypoxia increases the levels of hypoxia inducible factor-1 (HIF-1) protein and miR-210 in human MSCs (hMSCs). miR-210 positively regulates HIF-1α activity. Furthermore, miR-210 expression is also induced by hypoxia through the regulation of HIF-1α. To investigate the effect of miR-210 on hMSC survival under hypoxic conditions, survival rates along with signaling related to cell survival were evaluated in hMSCs over-expressing miR-210 or ones that lacked HIF-1α expression. Elevated miR-210 expression increased survival rates along with Akt and ERK activity in hMSCs with hypoxia. These data demonstrated that a positive feedback loop involving miR-210 and HIF-1α was important for MSC survival under hypoxic conditions.
|
|
|
KEYWORD
|
|
|
cell survival, human mesenchymal stem cells, hypoxia, hypoxia inducible factor-1, microRNA-210
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
   
|