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KMID : 0893420130140010095
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Journal of Veterinary Science
2013 Volume.14 No. 1 p.95 ~ p.98
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Serotype- and serogroup-specific detection of African horsesickness virus using phage displayed chicken scFvs for indirect double antibody sandwich ELISAs
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Wyngaardt Wouter van
Mashau Cordelia
Wright Isabel
Fehrsen Jeanni
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Abstract
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There is an ongoing need for standardized, easily renewable immunoreagents for detecting African horsesickness virus (AHSV). Two phage displayed single-chain variable fragment (scFv) antibodies, selected from a semi-synthetic chicken antibody library, were used to develop double antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISAs) to detect AHSV. In the DAS-ELISAs, the scFv previously selected with directly immobilized AHSV-3 functioned as a serotype-specific reagent that recognized only AHSV-3. In contrast, the one selected with AHSV-8 captured by IgG against AHSV-3 recognized all nine AHSV serotypes but not the Bryanston strain of equine encephalosis virus. Serving as evidence for its serogroup-specificity. These two scFvs can help to rapidly confirm the presence of AHSV while additional serotype-specific scFvs may simplify AHSV serotyping.
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KEYWORD
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African horsesickness virus, double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), phage-displayed scFv, serotype-specific
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