|
KMID : 0893420130140030241
|
|
Journal of Veterinary Science
2013 Volume.14 No. 3 p.241 ~ p.248
|
|
|
GC/MS analysis of high-performance liquid chromatography fractions from Sophora flavescens and Torilis japonica extracts and their in vitro anti-neosporal effects on Neospora caninum
|
|
Seo Hun-Su
Kim Kyoung-Hee
Kim Dae-Yong
Park Bong-Kyun
Shin Nam-Shik
Kim Jae-Hoon
Youn Hee-Jeong
|
|
|
Abstract
|
|
|
|
We analyzed alcoholic extracts of herbs possessing anti-neosporal activity against Neospora (N.) caninum. To identify the chemical components of Sophora (S.) flavescens and Torilis (T.) japonica associated with anti-neosporal activity, specific fractions were isolated by high-performance liquid chromatography (HPLC). In vitro activity of the fractions against N. caninum was then assessed. Gas chromatography/mass spectrometry (GC/MS) was used to identify and quantify specific anti-neosporal molecules in the herbal extracts. Almost all HPLC fractions of S. flavescens and T. japonica had higher levels of anti-neosporal activity compared to the not treated control. Active constituents of the extracts were sophoridane, furosardonin A, and tetraisopropylidene-cyclobutane in S. flavescens; 5,17-β-dihydroxy-de-A-estra-5,7,9,14-tetraene, furanodiene, and 9,12-octadecadienoic acid (Z,Z)-(CAS,1) in T. japonica.
|
|
|
KEYWORD
|
|
|
GC/MS, HPLC, Neospora caninum, Sophora flavescens, Torilis japonica
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
   
|
|