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KMID : 0893420130140030329
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Journal of Veterinary Science
2013 Volume.14 No. 3 p.329 ~ p.335
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Improved rat spinal cord injury model using spinal cord compression by percutaneous method
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Chung Wook-Hun
Lee Jae-Hoon
Chung Dai-Jung
Yang Wo-Jong
Lee A-Jin
Choi Chi-Bong
Chang Hwa-Seok
Kim Dae-Hyun
Chung Hyo-Jin
Suh Hyun-Jung
Hwang Soo-Han
Han Hoon
Do Sun-Hee
Kim Hwi-Yool
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Abstract
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Here, percutaneous spinal cord injury (SCI) methods using a balloon catheter in adult rats are described. A balloon catheter was inserted into the epidural space through the lumbosacral junction and then inflated between T9-T10 for 10min under fluoroscopic guidance. Animals were divided into three groups with respect to inflation volume: 20 μL (n = 18), 50 μL (n = 18) and control (Fogarty catheter inserted but not inflated; n = 10). Neurological assessments were then made based on BBB score, magnetic resonance imaging and histopathology. Both inflation volumes produced complete paralysis. Gradual recovery of motor function occurred when 20 μL was used, but not after 50 μL was applied. In the 50 μL group, all gray and white matter was lost from the center of the lesion. In addition, supramaximal damage was noted, which likely prevented spontaneous recovery. This percutaneous spinal cord compression injury model is simple, rapid with high reproducibility and the potential to serve as a useful tool for investigation of pathophysiology and possible protective treatments of SCI in vivo.
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KEYWORD
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balloon compression, laminectomy-free, magnetic resonance imaging, percutaneous spinal cord injury, rat
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